Creative Biogene provide support for your microbial fermentation projects to optimize your existing fermentation and purification processes, and improve manufacturability and process economy irrespective of your development stage or expression platform. Process optimization is key to reducing potential pitfalls, such as formation of product related impurities or improper folding, leading to insoluble aggregates. Our experience with upstream optimization techniques and materials allows us to produce a functional protein across a variety of molecule types. We have equipped upstream development laboratory to develop, optimize and scale a process. Downstream purification and refolding are conducted following fermentation. We have developed the unit operations of downstream processing sequence, speeding up development while maintaining the highest standard of quality. Process validation support activities increase the process understanding, identify critical process parameters and define operating ranges.

Strain Selection

Select a suitable microbial strain for the desired fermentation application. Consider factors such as product yield, metabolic capabilities, environmental requirements, and genetic stability.

Medium Formulation

Develop a growth medium that provides the necessary nutrients for microbial growth and product formation. The composition of the medium should support optimal cell growth and productivity. Factors such as carbon source, nitrogen source, minerals, vitamins, and growth factors need to be optimized for the specific strain and product.

Inoculum Development

Establish an efficient and consistent inoculum development process. This involves selecting appropriate pre-culture conditions, such as inoculum size, growth phase, and incubation conditions, to generate a robust and viable inoculum for large-scale fermentation.

Process Parameter Optimization

Identify and optimize critical process parameters such as temperature, pH, dissolved oxygen, agitation, and aeration. These parameters significantly influence microbial growth, metabolism, and product formation. Using statistical experimental design methods, such as Design of Experiments (DoE), can help efficiently screen and optimize process parameters.

Fermentation Kinetics

Characterize the growth kinetics and metabolic behavior of the selected strain under different process conditions. Monitor key parameters such as biomass concentration, substrate consumption, product formation, and byproduct formation over time. This data helps understand the fermentation kinetics and guides process optimization.